MM -glycerolphosphate and 1 tablet complete protease inhibitor cocktail (Roche) per 50 ml.
MM -glycerolphosphate and 1 tablet total protease inhibitor cocktail (Roche) per 50 ml. Protein extracts from 1.5 x 105 ESCs have been separated by 3-8 Tris-Acetate gels (NuPAGE? making use…