200 M, the cell survival rate, measured with MTT approach, has considerable difference (*P 0.05), compared using the normal manage group, but taking into consideration the formation of myocardial injury model, 400 M is chosen as the very best. As shown in Table 1.Result from the impact of distinctive action time of sodium hydrosulfite on cardiomyocytesNote: Examine to regular handle group, *P 0.05, evaluate to low-glucose culture option group, P 0.Table two: Outcome of your impact of unique action time of sodium hydrosulfite on Cardiomyocytes ?(X ?s)Experimental group Blank control group Low-glucose culture answer group 30 min 1h 2h 4h 24 h OD values 0.6100 ?0.03 0.5950 ?0.055 0.4170 ?0.075 0.3820 ?0.056*D 0.3260 ?0.032*D 0.2698 ?0.010*D 0.0830 ?0.05 Survival rate ( ) one hundred 97.54 68.36 62.63 53.44 44.23 13.Experimental outcomes show that within the identical concentration, as sodium hydrosulfite injury time increases, the injury to cardiomyocytes significantly increases and cardiomyocyte survival rate drastically decreases, plus the cell survival rate, measured with MTT strategy, has important distinction (*P 0.05), compared using the normal manage group. As shown in Table two.Result of protective effects[21] of ferulic acid on cardiomyocytes with hypoxia/reoxygenation injury[11-18]Note: Examine to typical handle group, *P 0.05, compare to low-glucose culture answer group, P 0.Experimental outcomes show that as ferulic acid dose increases, the cell survival price, measured with MTT approach, considerably increases, indicating that ferulic acid has protective effects on the hypoxia/reoxygenation injury caused by sodium hydrosulfite, meanwhile, Chinese constructive handle group and Western positive handle group have strong protective effects on injured cardiomyocytes, and high dose ferulic acid group has substantially higher protective effects than the optimistic handle groups.1-Hydroxy-7-azabenzotriazole uses As shown in Table three.165894-07-5 Purity Table 3: Outcome of protective effects of ferulic acid on cardiomyocytes with hypoxia/ ?reoxygenation (X ?s)Experimental group High-concentration ferulic acid group: (800 M) Medium-concentration ferulic acid group (400 M) Low-concentration ferulic acid group (200 M) Chinese good handle group Western good handle group Blank manage group OD values 0.PMID:24458656 3898 ?0.063* 0.3368 ?0.051* 0.3002 ?0.045* 0.3056 ?0.015* 0.3460 ?0.031* 0.2804 ?0.072 Injury inhibition price ( ) 37.89 20.66 eight.07 6.88 22.Outcome of protective effects of drug-containing plasma [22,23] on cardiomyocytes with hypoxia/ reoxygenation injuryExperimental final results show that high, medium and low dose ferulic acidcontaining plasma groups, Chinese good plasma group and Western good plasma group have protective effects on cardiomyocytes with hypoxia reoxygenation injury, along with the protective effects of ferulic acidcontaining plasma are superior to Chinese and Western optimistic control groups; as ferulic acidcontaining plasma concentration increases, the survival rate of cardiomyocytes has no dependence of concentration, plus the survival price inside the lowdose ferulic acidcontaining plasma group may be the highest. As shown in Table 4.Pharmacognosy Magazine | July-September 2013 | Vol 9 | IssueNote: Evaluate to normal handle group, *P 0.Outcome of effects of ferulic acid and its drug-containing plasma on the ldh activity in the myocardial cell culture supernatant and na +-k +-atpase and ca 2+-atpase activity inside the myocardial cellExperiments show that Compared with manage group, the LDH leakage of damage group boost,.