Ntrifuge for 15 min at 12,000 rpm. Supernatants have been fractionated by lowering SDS-PAGE.
Ntrifuge for 15 min at 12,000 rpm. Supernatants have been fractionated by reducing SDS-PAGE. pY397-FAK, FAK, pY118-paxillin, paxillin, and -actin had been detected by immunoblotting with the corresponding mAbs. Immunoreactive…